The Carbohydrate of Bovine Prothrombin

The inner carbohydrate was resistant to enzymatic attack and consisted of about one-third of the total mannose and one-half of the total iV-acetylglucosamine residues. It was shown that the carbohydrate chain was not attached to serine or threonine and that it is probably linked to asparagine. The cx and p anomeric forms of galactose were present in about a 1: 1 ratio. Glycopeptides containing CP and /3galactosidic bonds could be partially separated, indicating that the two anomers are incorporated into specific carbohydrate chains and are not randomly dispersed. The carbohydrate content and enzymatic degradation revealed few differences between the remainder of the carbohydrate in the CYand P-galactose-containing glycopeptides.